American Society for Clinical Pathology (ASCP) Histotechnician Certification Practice Exam 2025 - Free Histotechnician Certification Practice Questions and Study Guide

Question: 1 / 400

Which of the following statements regarding temperature and staining artifacts is true?

Higher temperatures always yield better results

Lower temperatures prevent all staining artifacts

Excessive heat can lead to staining artifacts

Excessive heat can indeed lead to staining artifacts, making this statement true in the context of histotechnology. When tissue samples are exposed to high temperatures during processing or staining, it can cause proteins to denature or alter their structure. This denaturation can result in uneven staining, excessive background staining, or loss of specific staining characteristics that are vital for correct diagnosis. High temperatures can also affect the binding of stains to tissue components, leading to artifacts that can confuse the interpretation of histological slides.

Maintaining the appropriate temperature during the staining process is essential to preserve the integrity of tissue samples and the specificity of stains used in histopathological evaluation. Thus, control of temperature is critical in laboratory practices to minimize staining artifacts and ensure accurate results.

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Staining artifacts are unrelated to temperature

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